Nanoparticles as vectors for antibiotic resistance: The association of silica nanoparticles with environmentally relevant extracellular antibiotic resistance genes
نویسندگان
چکیده
A relevant but yet unconsidered subset of particles that may alter the fate extracellular antibiotic resistance genes (eARGs) are nano-scale (NPs), which ubiquitous in natural environments and have unique properties. In this study, sorption isotherms were developed describing association linear DNA fragments isolated from widespread eARGs (blaI nptII) with either micon-sized kaolinite or silica nanoparticles (SNPs), to determine if capacity was enhanced at nanoscale. For each isotherm, eARG added five starting concentrations (5–40 μg/mL) mixed batch systems 0.25 g nuclease-free water. Sorption quantified by removal solution, as detected a Qubit fluorimeter. Isotherms for various fragment lengths (508, 680 861 bp), guanine-cytosine (GC) contents (34%, 47% 54%) both double single stranded eARGs, assess impact properties on particle association. also humic acid and/or CaCl2, these environmental parameters sorption. FTIR analysis performed analyze conformation sorbed eARGs. Desorption studied quantifying eDNA washed vortexed post-sorption particles. Statistically significant irreversible environmentally NPs (humic functionalized nanoparticles) demonstrated first time. Nano-emergent did not increase led compressed The addition acid, increased CaCl2 concentration small size favored showed slight preference single-stranded over double-stranded DNA. These findings suggest NP be phenomenon could influence spread resistance.
منابع مشابه
Implication of Biological and Synthetic Nanoparticles in Evolution and Development of Antibiotic Resistance Relevant for Aquaculture
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ژورنال
عنوان ژورنال: Science of The Total Environment
سال: 2021
ISSN: ['0048-9697', '1879-1026']
DOI: https://doi.org/10.1016/j.scitotenv.2020.143261